Health

A new mechanism of drug resistance discovered for breast cancer

Emergence of CSCs following IFN-I treatment. a, Multiparametric flow cytometry analysis of the illustrated CSC surface markers in MCA205 and AT3 cells treated with mock (CTR) or IFN-I (6 × 103 U ml–1, 72 h). Representative biparametric plots and histograms showing CD133+CD24+CD44+ percentages (mean ± s.e.m. with individual data point, n = 3 and n = 4 independent experiments) are shown. b, Flow cytometry analyses of CD44L and CD44H percentages (top) and qRT–PCR analyses of the reported TF (bottom) in FACS-isolated CD133 and CD133+ MCA205 cells treated as in a. Mean ± s.e.m. with individual data point, n = 3 independent experiments. qRT–PCR data are reported as mean fold change (FC) ± s.e.m. over CTR after Ppia intrasample normalization, n = 3 and n = 2 independent experiments. *P < 0.05, **P < 0.01, ***P c, SP (Hoechst 33342 within propidium iodide, PI) in MCA205 and AT3 cells left untreated (black), treated with VRP (100 μM, light green), IFN-I (blue) or VRP + IFN-I (dark green). Mean ± s.e.m. with individual data point, n = 9 and n = 6 independent experiments. d, TF expression levels in IFN-I-treated MCA205 cells. Data are reported as in b, n = 3 and n = 4 independent experiments. *P > 0.05, **P > 0.01, ***P > 0.001. e, Clonogenicity of MCA205 and AT3 cells plated in soft-agar upon treatment as in a. The number (mean ± s.e.m. and individual data point) of biologically independent samples collected over three independent experiments is shown. f, Ex vivo flow cytometry of CD44L and CD44H cells within the CD45 negative (CD45) fraction of MCA205 tumors from C57Bl/6J mice either treated with one single dose (1 × 105 U) or repeated doses (2 × 104 U) of IFN-I. Mean ± s.e.m. and individual data points for 10 mice per group from two experimental replicates. a,b,d Unpaired two-sided Student’s t-test and unpaired two-sided Student’s t-test with Welch’s correction compared with CTR. c,f, Brown–Forsythe test with Dunnet’s correction and ordinary one-way ANOVA test followed by Bonferroni’s correction. e, Unpaired two-sided Student’s t-test with Welch’s correction and two-tailed Mann–Whitney test compared with CTR. Credit: Nature Immunology (2022). DOI: 10.1038/s41590-022-01290-3

Scientists from Università Cattolica in Rome, together with colleagues from the IIGM Foundation (part of Candiolo Institute at Turin), have discovered a new drug-resistance mechanism in breast cancer that leads to the formation of cancer stem cells, (the cells that feed the tumor and cause relapse and metastasis). They have also devised an experimental therapy to bypass or prevent the emergence of drug-resistance.

The results are published in Nature Immunology. The research was coordinated by Antonella Sistigu and Martina Musella, Department of Translational Medicine and Surgery, section of General Pathology and Clinical Pathology directed by Professor Ruggero De Maria and Ilio Vitale of the IIGM Foundation—Italian Institute for Genomic Medicine (part of the Compagnia di San Paolo Foundation), at Candiolo Institute, FPO-IRCCS, Candiolo (Turin).

Italian scientists discovered how the tumor evolves during treatment and develops resistance to therapies. “More specifically,” Sistigu and Musella explain, “we have demonstrated that some tumor cells, while dying as result of chemotherapy, release in the tumor microenvironment a group of factors called ‘alarmins,’ which, normally alert and activate the immune system. However, paradoxically, some of these alarmins, such as type I interferons, can reprogram residual cancer cells and transform them into cancer stem cells, the deadly reservoir of the tumor. These stem cells are responsible, for example, for disease recurrence and metastasis.”

Cancer stem cells escape the control of the immune system and have high invasive and aggressive potential.

Italian scientists have discovered that this mechanism of drug resistance depends on activation of a protein called “KDM1B”. KDM1B controls and regulates gene expression.

After the discovery in animal models, Ilio Vitale explains, “we studied 5 different cohorts of patients and confirmed that this mechanism is valid also in patients.”

Finally in laboratory experiments the scientists saw that inhibition of KDM1B prevented cancer stem cells formation and increased the effectiveness of therapy.

“Based on these results we propose a combination therapy (some specific chemotherapeutics and immunotherapeutics, together with the experimental drug that inhibits KDM1B, to prevent formation and possibly effectively target this subpopulation of stem cells otherwise resistant to any treatment,” the researchers say.

The next step of this research will be to evaluate the combination therapy in trial clinic on patients, Sistigu concludes.


Gene targeting overcomes the resistance of brain cancer to therapy


More information:
Martina Musella et al, Type I IFNs promote cancer cell stemness by triggering the epigenetic regulator KDM1B, Nature Immunology (2022). DOI: 10.1038/s41590-022-01290-3

Provided by
Universita Cattolica del Sacro Cuore

Citation:
A new mechanism of drug resistance discovered for breast cancer (2022, October 6)
retrieved 6 October 2022
from https://medicalxpress.com/news/2022-10-mechanism-drug-resistance-breast-cancer.html

This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no
part may be reproduced without the written permission. The content is provided for information purposes only.



Most Related Links :
UK Prime News Latest News Sports News Finance News Automobile News

Root link

Related Articles

Back to top button